dextran hydrodynamic radius

Using the polymer standards, a plot of the ln [] vs ln Mw usually gives a straight line. J Appl Toxicol. FOIA (1954). Kasaai, Mohammad R. (2012). Curvale, R.; Masuelli, M.; Perez Padilla, A. 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. Dextran. Pig skins (Micropig Franz cell membrane) were supplied by Medi Kinetics Co., Ltd. (Pyeongtaek, Korea). Carbohydrate Polymers, 36, 319-326. Format), Citation-(BibTeX From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. 2014 Sep;5(9):2324-30. doi: 10.1039/c4fo00319e. As a consequence of the diversity in their origin, the set of classical universal size independent quantities suffers from shortcomings. Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. Coviello, T.; Matricardi, P.; Marianecci, C.; Alhaique, F. (2007). These results suggest that fibroblasts proliferate and migrate under the SC, and regenerate the skin. Pharmacol Pharm. 10K, and c M.W. The Mark-Houwink. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. The skin was treated with the RF-based microporation device, inducing micropore ablation through alternating electrical current. Exp Cell Res. 2012;27(3):20212. Format), Citation-(EndNote Critical parameters for gel permeation WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. The calculation of Mark-Houwink parameters is carried out by the graphic representation of the following equation: where kMH (cm3/g) and a are Mark-Houwink constants, depending on the type of polymer, solvent, and temperature of intrinsic viscosity determinations [46]. Saudi Pharm J. Each microporated sample was incubated in the skin culture medium. The great deviation at 2000kDa occurs due to the large hyperbranched structure of the macromolecule, generating a change in its conformation and, therefore, a change in the way it flows [73]. Kasaai, Mohammad R. (2007). Thus, it is a common practice to express the frictional coefficient of rigid structures as. Arch Dermatol Res. The basic setup of a DLS instrument is shown in Figure 1. Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. Polymer molecular weight distribution. Dependence on Concentration. Unauthorized use of these marks is strictly prohibited. The analysis of errors using the Solomon-Ciuta method is compared with HM [59]. Webof overall macromolecular geometry and hydrodynamic draining. ; Yevlampieva N.P. Recent developments in polysaccharide-based materials used as adsorbents in wastewater treatment. Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. Corp. (Seoul, Korea). Pathan IB, Setty CM. (2008). In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)). Cookies policy. pp13-21. Drug deliv. Figure5 shows the cumulative amount of the FITC-dextran (M.W. Gillespie, T.; Hulme, M. A. Carbohydrate Polymers, 39, 377-380. Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. Pharmaceutical Quality Dextran 40 is available from Pharmacosmos as active pharmaceutical ingredients (API) as excipient for final formulation, drug product, for medical devices, for production of solutions for injection and infusion and much more. The effect of branching is best recognized from the molecular weight dependencies of []. ; Weisz, J. Armstrong, J. K.; Wenby, R. B.; Meiselman, H. J.; Fisher, T. C. (2004). Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named. eCollection 2015. Data File Dextran. European Polymer Journal, 37, 619-622. High Manufacturing Standards of Molecular Probes Dextrans. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. (2008). Food Sci Biotechnol. Webwhittier union high school district superintendent. Carbohydrate Polymers, 74, 372-378. The Intrinsic Viscosity of Biological Macromolecules. Quantitative data are presented as the meanstandard deviation, and comparisons were carried out using one-way ANOVA (Systat Software Inc., Chicago, IL, USA). Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. Expert Rev Med Devices. Senti, F. R.; Hellman, N. N.; Ludwig, G.; Babcock, E.; Tobin, R.; Class, C. A., (1955). where c is the solute concentration (g/cm3), [] is the intrinsic viscosity (cm3/g), and kH represents Huggins constants (cm3/g). Careers. Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. Hindered diffusion of dextran and ficoll in microporous membranes. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. (2000). Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. Dilute Solution. 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. The important role of thermodynamic interaction parameter in the determination of theta temperature, dextran/water system. Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. Journal of American Chemical Society, 76 (20), 5041-5052. Biomaterials, 22, 1891-1898. Working with the molecular weights given by the manufacturer and compare them with the molecular weights, calculated from the Mark-Houwink parameters in this work; can be seen that the %RE obtained, see Table 6. The a value is higher than calculated in this work, because these calculations were performed with number average molecular weight (Mn) which rearranges the value of a and forces him to enter into unbranched or linear molecules (a > 0.5), on the other hand, if molecular weights (Mw) ware used to value would be less than 0.5. 2023 BioMed Central Ltd unless otherwise stated. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. Prausnitz MR, Langer R. Transdermal drug delivery. Biomater Res 24, 22 (2020). Dextrans in Aqueous Solution. Im Tiefkhlgert (-5 bis -30C) lagern und vor Licht schtzen. In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. Journal of Applied Polymer Science, 27, 795-810. Solution properties of branched dextran. The micropores of the human cadaver skin were regenerated according to the time. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. Polymolecularity and polydispersity in Molecular weight determinations. Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost ACS Nano. (2008). (1998). Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. Normally, the skin loses very little water loss whereas damaged skin loses more [22]. Sci. ; Aberle, T; Burchard, W. (2000). 4K and M.W. The mechanism of the dextran-induced red blood cell Aggregation. Viet, D.; Beck-Candanedo, S.; Gray D. G. (2008). The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. 2013;43(4):287303. As well as being able to measure hydrodynamic radius in the Zetasizer (if you have access to one), it's also provided with a calculator that allows Molecular weight, particle size and diffusion coefficient can be determined in many studies by Dynamic Light Scattering [20, 21, 22], Gel Permeation Chromatography [23], Analytical Ultracentrifuge [24, intrinsic viscosity [1, 7, 14], size exclusion chromatography / multiangle laser light scattering / differential viscometry, Flow Field-Flow Fractionation [25], and so on. Microneedles and their applications. The lack of data on the uniformity of intrinsic viscosity measurements in the system water/dextran, highlights the significant influence of the solvent and temperature [74]. Morris, G.A. The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. FITC-dextran solution (600L) was added to the donor chamber. Solvent effects on polysaccharide conformation. The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. International Journal of Biological Macromolecules, 42, 133-137. Kirkland, J. J.; Dilks, C. H.; Rementer, S. W. (1992). The intrinsic viscosity calculation requires several concentrations, and because of this inconvenient will not use the Huggins method in this paper. Ther. Bahary, W.S. In our present study, microporated skin permeated insufficient amount of 20K FITC-dextran, suggesting that enhancement of permeability was unsuccessful with macromolecules of more than 20KM.W.. In the future, we plan to investigate the possibility of enhancing the delivery of other macromolecular drugs such as peptides and siRNAs using the RF-based microporation device. The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. Antoniou, E.; Buitrago, C. F.; Tsianou, M.; Alexandridis, P. (2010). Keywords: The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer. 2013;3(5):3588605. Synthesis of dextran by dextransucrase treated, sucrose-supplemented skimmed milk revealed the promising potential of dextran as a food additive. The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. The hydrodynamic radius of 2-NBDG glucose was too small to measure. Materiaux 1-5. ; Bataille, B. A similar combination involves the intrinsic viscosity and specific volume: (a/b) is called Einstein viscosity increment, and Vs is specific volume (cm3/g). These results indicate that healing and regeneration occurred in the human cadaver skin after the microporation. Intrinsic viscosity-molecular weight relationship for chitosan. This result could reflect the presence of some aggregated species within the lysozyme sample solution. Google Scholar. DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. Ioan, C.E. WebIn this regard, we chose as crowding agents a number of polymers with hydrodynamic dimensions comparable to those of iRFP713 in different structural states: polyethylene glycol8,000(PEG-8000)withaneffectivehydrodynamicradiusof24.5 1.9(Devanand & Selser, 1991), Dextran-40 and Dextran-70 with hydrodynamic radius of 44.550 and 5864 , Durand, A. (2007). 24h after microporation, the pore sizes reduced from 64.8817.20m (0h) to 48.854.27m (24h), and the pore depths reduced from 83.9120.56m (0h) to 63.9527.61m (24h). There is a formula that used to calculate the radius (in Angstrom) from the molar mass (in g/mol) of dextran, a=0.33*(MM^0.46) where, a and MM are The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). The authors declare that they have no competing interests. Bookshelf official website and that any information you provide is encrypted Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). Carbohydrate Polymers, 79, 380-390. Citation-(RIS GYA primarily conducted research. The human skins were from the back or thigh of a 24-year-old woman. The cumulative permeation of FITC-dextran 10K was 4.820.32M, in the human skin and 5.920.46M in the pig skin. With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. Int J Food Sci Nutr. For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. There is a deviation from linearity after 580kDa [69, 70]. 20K. * shows a significant difference between the M.W. Pore closure examination was performed using an optical microscope (BX43, Olympus, Japan) immediately (time 0), 4, 8, and 24h following device application. The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. Karmarkar, S.; Garber, R.: Kluza, J.; Koberda, M. (2006). This device can generate microchannels from the SC through to the outer dermis and allow transdermal delivery of active agents. ; Ross-Murphy, S.B. Intrinsic viscosity of bovine serum albumin conformers. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. Journal Non-Newtonian Fluid Mech., 97, 295-301. Journal of Applied Polymer Science, 72, 871-876. Pharmaceut Sci Tech. Molecular weight, molecular weight distribution and molecular size of a native dextran. Charge: Anionic. Table1 shows TEWL values measured immediately after the microporation of human and pig skins. (2001). ; Talsma, H.; Hennink, W.E. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. Google Scholar. He, L.; Niemeyer, B. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. osmotic pressure or light scattering). (2003). Walkenhorst, S.; Olivier, R. (2002). Liu Zonghua; Yanpeng Jiao; Yifei Wang; Changren Zhou; Ziyong Zhang (2008). Journal of Physical Chemistry, 58(11), 953-957. The micropore formed at the SC through to the epidermis, enabling drug delivery. Molecular weight (M v) and hydrodynamic properties can be calculated from there. For a given polymer-solvent pair, the theta condition is satisfied at a certain temperature, called the theta () temperature or theta point. Ibrahim O, Munavalli GS, Dover JS. Herwadkar A, Banga AK. Studies carried out at different ionic strength you can see them in the references [64, 65] and works with different solvents in the references [66, 67]. ; Fran, H.P. Kuge Takashi; Kimie Kobayashi; Shinichi Kitamura; Hiroshi Tanahashi. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. Monolayers and Thin Films of Dextran Hydrophobically Modified. J Pharm Sci. (2003). Lpez Martnez, M. C.; Daz Baos, F. G.; Ortega Retuerta, A.; Garca de , J. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. Where f0 is the frictional coefficient of a sphere having the same hydrodynamic (hydrated or solvated) volume V as the particle. WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Expert Opin Drug Deliv. Catiker, E.; Guner, A. For a limited temperature range, the viscosity of a polymer solution varies generally following a relation similar to that of usual liquids (rrhenius plot), where the polymer concentration is not very high and that temperature remains far enough from glass transition [38], and for system PEAorganic solvents see reference [63]. In summary, we demonstrated effective enhancement of skin permeability and delivery of macromolecules lower than 20KM.W., and regeneration of human cadaver skin after microporation with an RF-based microporation device. Carbohydrate Polymers 87, 405-410. Journal of Controlled Release, 119, 5-24. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect. Human cadaver skin has been confirmed as alive skin tissue through analysis of its morphology and enzymes, and its usefulness as an alternative skin membrane in drug permeation experiments has been verified [20]. ; Hogan, M.P. Progr. Not for use in diagnostic procedures. In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). WebDextran 40 Pharmaceutical Quality. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). 2012;101(8):28619. Epub 2021 Feb 9. Mainaa, Ndegwa Henry; Pitknen, Leena; Heikkinen, Sami; Tuomainen; Pivi; Virkki, Liisa; Tenkanen, Maija (2014). Transdermal drug delivery system for non-steroidal anti inflammatory drugs: a review. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). An undergraduate physical chemistry experiment. (1999). (2013). Just in case anyone wants to use a different molecular weight to estimate the hydrodynamic size: the calculator is integrated into the classic Zeta Dextran is soluble in water, methyl sulphoxide, formamide, ethylene glycol, glycerol, 4-methylmorpholine-4-oxide, and hexamethyl phosphamide [4]. Sachdeva V, Banga AK. (2003). (2013). Biomacromolecules 8, 2464-2475. Pharm Res. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. Amersham Biosciences, AA Edition, www.amershambiosciences.com. For a values from 0-0.5 a rigid sphere is predicted in a ideal solvent, from 0.5-0.8 a random coil in good solvent, and from 0.8-2 a rigid or rod like shape is expected (stiff chain). (2004). The Transdermal diffusion cell system was protected from light.

Permanent Jewelry Florida, John Hays Obituary, Why Does My Corn On The Cob Have Red Spots, Florida Fourth District Court Of Appeal Judges, Articles D